Exploring inhibitory neuron-secreted chemokines as a novel remyelination therapy – Post-doc or student position, Voronova lab, U Alberta

Neural precursor cells in adult mammalian brain, such as subventricular zone (SVZ) neural stem cells and oligodendrocyte precursors in brain parenchyma, form oligodendrocytes throughout life. The purpose of oligodendrocytes is to produce myelin, an insulating material that performs vital functions in efficient neural information transmission and constitutes the brain white matter. Demyelinating disorders occur when myelin, a protective substance that coats nerve projections and ensures efficient neuronal signalling, is damaged. This leads to nerve dysfunction and neurological impairments. Demyelination occurs in neurological disorders such as multiple sclerosis (MS), in which myelin is attacked and damaged by the body’s own immune cells. Current MS treatments only target inflammation but not central nervous system (CNS) remyelination, which could be achieved by stimulating the production of new oligodendrocytes.

Postdoctoral fellow or Ph.D. candidate will investigate how developmentally important neuronal chemokines (Voronova et al. 2017 Neuron) affect precursor-microglia cell-to-cell interactions for enhanced remyelination. The production of oligodendrocyte lineage cells and/or new myelin will be investigated using transgenic animals. Other techniques will include live imaging, flow cytometry, phagocytosis assays, brain slice and primary cell culture, intracerebroventricular injections (live animal surgery), RNA-sequencing and in situ hybridization, cytokine analysis, immunohistochemistry as well as confocal and electron microscopy.

An ideal candidate will be highly motivated and can work as a team player and a leader. Preference will be given to applicants with previous rodent experience and/or extensive cell or developmental biology expertise. Highly motivated Ph.D. and postdoctoral fellow candidates ready to learn live animal surgeries and work collaboratively and responsibly are encouraged to apply. Salary for potential postdoctoral fellow with commensurate with experience. Position will be open until filled.

Candidates should submit:
i) cover letter including research interests including a brief summary of previous graduate thesis and/or post-doctoral research (if applicable);
ii) complete CV;
iii) transcripts (unofficial is suitable); and
iv) the names and contact information of at least two academic referees.

E-mail applications to voronova@ualberta.ca. Only applicants selected for an interview will be contacted.

Download a PDF version of this advertisement: 20190522-Voronova

Graduate Student/Postdoctoral Positions in Cellular Neuroscience – Bowie lab, McGill University

Two CIHR-funded positions are available immediately for enthusiastic graduate students/postdoctoral fellows with a keen interest in using electrophysiology and imaging techniques to study ligand-gated ion-channels and their role in neuronal circuit behavior.

The first project will focus on an ongoing theme to understand the structure-function properties of ionotropic glutamate receptors. The successful applicant will use single-channel and macroscopic patch clamp techniques to tease apart the mechanism of channel activation in wildtype and mutant receptors. This work will be part of a multilab collaboration with researchers using computational chemistry and structural biology.

The second project will examine the signaling defects associated with Fragile X syndrome, a prominent neurodevelopmental disorder. The successful applicant will study neuronal circuit connectivity using single and multi-electrode recordings from acutely isolated brain tissue. Imaging and functional experiments will be collected simultaneously using a multiphoton microscope equipped with an electrophysiology rig.

Related Publications
Dawe et al (2016) Distinct structural pathways coordinate the activation of AMPA receptor-auxiliary subunit complexes. Neuron.

Accardi et al (2014) Mitochondrial reactive oxygen species regulate the strength of inhibitory GABA-mediated synaptic transmission. Nat Commun.

*Student stipends will be based on the policy recommendations of individual McGill Graduate Programs. Postdoctoral salaries will be based on CIHR recommendations and level of experience*

Applicants should send a cover letter, cv and the names of 2-3 referees to:

Dr. Derek Bowie,
Department of Pharmacology & Therapeutics
McGill University, Faculty of Medicine
3649 Promenade Sir-William Osler, Rm 164
Montreal, QC, Canada, H3G 0B1

Tele: 514-398-1581 Email: derek.bowie@mcgill.ca
Webpage: http://www.medicine.mcgill.ca/pharma/dbowielab

Connect on LinkedIn: https://www.linkedin.com/in/derek-bowie-177a7855/

Download a pdf version of this advertisement: Bowie-Advertisement-PDF

Study the Basic Mechanisms of Multiple Sclerosis: Graduate Student & Postdoctoral Fellow Positions Available (University of Saskatchewan)

Join a dynamic, world-class, generously funded basic and translation science team. Located in state-of-the-art laboratory facilities in the Saskatoon City Hospital, basic scientists are able to work hand-in-hand with clinicians from the Saskatoon MS Clinic (also located in Saskatoon City Hospital). Graduate Student and Postdoctoral Fellow positions in the Office of the Saskatchewan MS Clinical Research Chair are offered highly competitive compensation, personalized mentorship with in vitro, in vivo, genetic, and human projects available.

Contact information Ms. Colleen Cochran, colleen.cochran@usask.ca

Graduate Student positions (PDF)

Postdoctoral Fellow positions (PDF)

TWO Graduate student positions in Neural Stem Cell Biology & Neural Stem Cell Regeneration – LINDSEY LAB – University of Manitoba

Graduate student position in Neural Stem Cell Regeneration

The Lindsey Lab is looking for a highly motivated student to fill an open funded graduate student position for September 2019. The focus of the project will be on the cellular and molecular regulation of neural stem cells during the regenerative process following CNS injury in the zebrafish model. The Lindsey Lab is the first laboratory at the University of Manitoba to establish the zebrafish model, and one of the only laboratories in Canada investigating CNS neural stem cell repair using this experimental system. Unlike in mammals, the zebrafish brain remains highly regenerative throughout life. This is made possible by distinct populations of neural stem cells with the potential to generate newborn neurons. The exceptional regenerative capacity of the zebrafish has made it a mainstream vertebrate model to uncover fundamental mechanisms of neural repair for the development of novel approaches to regenerative medicine. To date however, the interplay between the innate molecular programs of regenerative neural stem cells, the niche micro-environment, and the immune response, that together promote successful adult CNS regeneration is poorly understood. We are interested in characterizing the cellular and molecular regulators of neural stem cell populations post-injury to better understand how we may harness the regenerative potential of zebrafish neural stem cells for human therapies related to neurotrauma and neurodegenerative disease.

Graduate student position in Neural Stem Cell Biology

The Lindsey Lab is looking for a highly motivated student to fill an open funded graduate student position for September 2019. The focus of the project will be on neural stem cell niche development in the zebrafish model. The Lindsey Lab is the first laboratory at the University of Manitoba to establish the zebrafish model, and one of few laboratories in Canada investigating CNS neural stem cell development and plasticity using this experimental system. In recent years, the zebrafish has become a leading model for examining neural stem cell behavior and plasticity. The transparency of zebrafish larvae along with the abundance of lifelong stem cell compartments makes this model highly amenable to tracking stem cell niche growth, maintenance, and senescence.

The vertebrate adult neurogenic niche is composed of heterogeneous stem cell populations that receive cues from the vasculature, adjacent neurons, and ventricular cerebral spinal fluid. How adult neural stem cell niches of the vertebrate brain are constructed over development however, currently remains poorly understood. We are interested in defining how diverse stem cell niches are established from the larval to adult brain, and the cellular (i.e. cell cycle changes, differentiation, apoptosis, senescence) and molecular (i.e. signaling pathways, genes, growth factors) drivers involved in this process.

About the Lindsey Lab

The overarching research focus of the Lindsey Lab is to understand context-dependent behavior and control of distinct neural stem cell populations during development, with environmental interactions and learning, and repair. We use a combination of modern cellular and molecular approaches, such as proliferation and senescence assays, lineage tracing, in situ hybridization, transgenic animals, immunohistochemistry, RNA-sequencing, and injury models. These techniques are coupled with advanced 2-dimensional (live or fixed fluorescence confocal microscopy, electron microscopy and correlative electron microscopy) and 3-dimensional (optical projection tomography, confocal/light-sheet microscopy, tissue clearing) imaging. Finally, we use specialized behavioural software to understand the relationship between animal behavior/neurotrauma – stem cell dynamics – and neurogenesis/neuro-regeneration.

Students joining the Lindsey Lab can expect high quality research training and mentorship in a supportive and collaborative learning environment. Trainees will have the opportunity to present their work at local, national, and international scientific meetings and collaborate with diverse researchers within the Rady Faculty of Health Sciences. A highly competitive graduate student stipend is available for the successful candidate. However, all students will be expected to apply for local and national level graduate student scholarships during tenure of the position.

The Lindsey Lab is located within the Department of Human Anatomy and Cell Science in the Rady Faculty of Health Sciences at the University of Manitoba in Winnipeg. http://umanitoba.ca/faculties/health_sciences/medicine/units/anatomy/12818.html

How to apply

Interested candidates should send a brief statement of research interests, a copy of their most recent transcript, and their CV to Dr. Benjamin Lindsey at Benjamin.lindsey@umanitoba.ca. If shortlisted, potential candidates will be asked to provide contact information for three references. Students with an interest in fundamental neural stem cell behavior are highly encouraged to apply.

PhD position at Université Laval: Unveiling the role of protein aggregation in Parkinson’s disease and neurodegenerative disorders

A PhD position in Neuroscience/neurophotonics is available at the Faculty of Medicine, Université Laval (https://www.ulaval.ca/) in Quebec City, Canada (https://www.ville.quebec.qc.ca/en/).

The selected candidate will work on a collaborative project involving the laboratory of Dr. Abid Oueslati (https://abidlncf.wixsite.com/oueslati-laboratory) and the laboratory of Dr. Armen Saghatelyan (https://www.saghatelyanlab.com/research). This project aims to understand the role of protein aggregation, notably alpha-synuclein aggregation, in the pathogenesis of Parkinson’s disease and related neurodegeneration. The project will be essentially based on using a multidisciplinary approach, including optogenetic, calcium imaging in feely moving animals, two-photon imaging, brain tissue CLARITY and Light Sheet Fluorescence Microscopy.

The desired applicant must have a master in neuroscience or cell biology, or the equivalent, in addition to a strong academic record.

Contact information:

Interested applicants should submit a letter of interest, curriculum vitae and the names and addresses of 2-3 references to Dr. Oueslati (Abid.Oueslati@crchudequebec.ulaval.ca) and Dr Saghatelyan (armen.saghatelyan@fmed.ulaval.ca).

Positions available-Neurobiology of Pain– Kerr laboratory, University of Alberta

The Kerr laboratory at the University of Alberta is recruiting for graduate students (MSc or PhD) to study the role of the peripheral nervous system in central neuropathic pain states with an emphasis on animal models of MS. We are looking for candidates with an interest in behavioural neuroscience but with some background in cell and molecular biology. Skills in electrophysiology would be an asset.

Please send a CV with a statement about research interests and up to date transcripts to:
bjkerr@ualberta.ca

Contact information

Dr. Bradley Kerr
Department of Anesthesiology and Pain Medicine, CSB 2-150
University of Alberta
Edmonton, Alberta, Canada T6G-2G3
Email: bjkerr@ualberta.ca
Website: https://amsrguofa.ca/

Posting end date 2019/08/30

Grad student with Dr. Jason Plemel: understanding regeneration associated microglia – University of Alberta

A grad student position is available in the lab of Dr. Jason Plemel. The lab is focused on understanding how one cell, microglia, can be both important in promoting regeneration, but also a contributor to neurodegenerative conditions. The Plemel lab uses tools like transgenic mice, in vivo models of white matter injury, flow cytometry, cell culture and single-cell RNA sequencing to understand how microglia behave in disease. Start dates are negotiable, but we would like a new student in by September 2019. Students interested in neurological disorders, especially those white matter diseases like Multiple Sclerosis, are encouraged to apply. Please send an updated CV, transcript (unofficial is suitable) and contact information for two academic references to jrplemel@ualberta.ca. To see the website, please visit (https://amsrguofa.ca).

Contact information Jason Plemel (Jrplemel@ualberta.ca)

Posting end date:  2019/08/30

PhDs in Neuroscience/Neuropharmacology – CIHR funded (5 years) Fernyhough Albensi labs, University of Manitoba

DIVISION OF NEURODEGENERATIVE DISORDERS
ST. BONIFACE HOSPITAL ALBRECHTSEN RESEARCH CENTRE

University of Manitoba
PhDs in Neuroscience/Neuropharmacology – CIHR funded (5 years)

The St Boniface Hospital Albrechtsen Research Centre offers a vibrant and interactive research environment for graduate studies in the molecular cell biology of neurodegenerative diseases. Three PhD positions are available in the Pharmacology Graduate Program in the Division of Neurodegenerative Disorders:
Position 1: Role of the calcium channel, TRPM3, in controlling axonal outgrowth and repair. (PI: Dr. P. Fernyhough)
Position 2: Mechanism of muscarinic receptor regulation of mitochondrial function in axons of adult neurons. (PI: Dr. P. Fernyhough)
Position 3: Mitochondrial Dysfunction in Alzheimer’s Disease. (PI: Dr. B. Albensi)
Potential applicants for positions 1&2 can derive background information from Calcutt et al (2017) J.Clin.Invest. 127:608. For position 3, applicants should refer to Cadonic & Albensi (2016) Mol Neurobiol 53:6078 and Djordjevic et al. (2017) Neuroscience 361:81.
Students with an undergraduate and/or masters background in biology, cell biology, biochemistry, neuroscience and/or pharmacology/physiology are encouraged to apply. Stipends of CA$20,000 or more are available commensurate with level of experience.

Contact information

Interested students should send a CV to Dr. Paul Fernyhough (e-mail: pfernyhough@sbrc.ca) or Dr. Benedict C. Albensi (email: balbensi@sbrc.ca).

Posting end date 2019/03/31

Graduate student position in Pericyte Biology- Stobart Lab- University of Manitoba

Neuronal energy demand during synaptic activity is met by increasing local blood flow, a process which is known as functional hyperemia or neurovascular coupling.  Though extensively studied in recent years, the cellular mechanisms controlling functional hyperemia remain unclear.  Majority of the field has focused on interactions between neurons, astrocytes and arterioles, but new evidence suggests that vascular pericytes may regulate cerebral blood flow at the capillary level.  We have developed in vivo imaging techniques to simultaneously monitor pericyte calcium and blood flow. The proposed project will employ novel mouse models and these imaging techniques to investigate the effects of pericyte signaling on brain capillary blood flow in vivo.  There will be opportunities to learn mouse handling, stereotaxic injection of AAVs, in vivo two-photon calcium microscopy, intrinsic optical blood flow imaging, histological techniques and computer image analysis.

 

We are looking for motivated candidates who hold a B.Sc. in biology or related discipline.  They must be interested in neurobiology, have good problem solving skills, and can work independently.

Please submit a letter with statement of research interests, CV including research experience, official transcripts and contact details for 2 references by email.

 

Dr. Jill Stobart
jillian.stobart@umanitoba.ca
Assistant Professor
College of Pharmacy
University of Manitoba
Apotex Centre
750 McDermot Ave.
Winnipeg, Manitoba

Canada

CIHR-funded PhD position – Parent lab, Université Laval

The Parent lab is looking for a highly motivated and talented PhD student to fill an opened CIHR-funded position. Selected candidate will work to unravel the microcircuitry of the basal ganglia in Parkinson’s disease using transgenic mice, non-human primates and post-mortem human brains. Selected candidate will join a dynamic group at the CERVO Brain Research Centre affiliated with Laval University and located in Quebec City.

Interested candidates should send their CV, a statement of research interests as well as information for 2 references to:

Dr. Martin Parent
Research Group Leader
CERVO Brain Research Centre
2601, Canardière
Quebec City, G1J 2G3
Email: martin.parent@fmed.ulaval.ca
Phone: 418-663-5000 x6736