Research Assistant – Quandt lab – University of British Columbia

We have a position in our laboratory for a research assistant with funding for up to three years. The UBC posting can be found at:

Interested applicants are invited to review our website for more information.

Training & expectations: Major areas of focus will include inflammatory mediators, oxidative stress, environmental stressors, immune populations, neurodegeneration and repair. The successful candidate will have a solid understanding and demonstrated proficiency in several of the following and others will be provided on the job:

  • Cell culture and aseptic technique, preferably involving both isolation and maintenance of rodent primary cultures as well as cell lines.
  • Aseptic technique and experience with human blood handling for the isolation of PBMC and subsequent culture/assay
  • Ability to perform and troubleshoot a wide variety of molecular and cellular biology techniques, including many of the following:
    RNA/DNA isolation, quantitative PCR, transfection, siRNA knockdown and cDNA construct knockin, flow cytometry, immunohisto- and cytochemistry on cells as well as frozen and formalin-fixed, paraffin embedded tissues, fluorescence and light microscopy – conventional and/or confocal; ELISA, protein isolation and western blotting;
  • Willingness to work with small rodents is essential. Able to carry out and oversee procedures involving small rodents including immunization/injections, necropsy, blood sampling, and minor surgical procedures involved in animal surgery; applicants with experience in pre-clinical models of neurodegenerative diseases including MS are especially encouraged to apply.
  • Maintenance/monitoring of colonies of transgenic animals, including colony breeding, screening and management.
  • Excellent verbal and written communication
  • Understanding of experimental design and assay optimization is important along with ability to work well with others and to work independently.