Date: Saturday May 18, 2024, 11-4pm.
Location: Room 3402ABC, Djavad Mowafaghian Centre for Brain Health, 2215 Wesbrook Mall Vancouver, BC V6T1Z3
Estimated Attendance: 30-50 participants.
Registration Deadline: Participant must register before May 10, 2024
Registration Fee: Free; Registration is required for participant.
Food and Beverage: Coffee, water, and lunch will be provided.
Registration link: https://ubc.ca1.qualtrics.com/jfe/form/SV_1zgWJQljIgih5Sm
Organizers: Jeffrey LeDue1 , Timothy Murphy1 , and Majid Mohajerani2
- Djavad Mowafaghian Centre for Brain Health, University of British Columbia
- Douglas Research Centre, McGill University
Description: Linking brain circuit structure, neural activity with behaviour rests on modern data acquisition methods and experimental techniques relying on sophisticated tissue clearing and imaging techniques, next gen neural activity recording systems (e.g. ultra-wide field 2-photon microscopes, wearable microscopes, etc.) and synchronized video recordings of animal behaviours. Subsequent analysis of these data relies on many packages. Here we aim to explore through hands-on examples:
- Pose Estimation with Deeplabcut: github.com/DeepLabCut/DeepLabCut
- Calcium activity analysis with Suite2p: github.com/MouseLand/suite2p
- Whole brain analysis of cleared tissue with BrainQuant3d: github.com/sunilgandhilab/brainquant3d
The target audience of the workshop will be graduate students, postdoctoral researchers and principal investigators in neuroscience and psychology with interest in data analysis. Participants will perform the computer exercises using test data sets and analysis software loaded on their own laptop computers and will be led by senior graduate students and STEM undergraduate facilitators (from UBC and McGill). CAN membership or registration in the CAN 2024 meeting is not required to attend this workshop.
Program:
11.00-12.30 Pose Estimation with Deeplabcut
12.30-13:30 Lunch and discussion
13.30-15.00 Calcium activity analysis with Suite2p
15.00-16.00 Whole brain analysis of cleared tissue with BrainQuant3d