| Abstract No.: | C-D3150 |
| Country: | Brazil |
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| Title: | PARTICIPATION OF GLUTAMATERGIC SYSTEM AND PROINFLAMMATORY CYTOKINES ON THE ANTINOCICEPTIVE ACTION OF A CRUDE EXTRACT OF ROOTS FROM Pfaffia glomerata(SPRENG) PEDERSEN IN MICE |
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| Authors/Affiliations: | 2 Cristiane Hatsuko Baggio*; 2 Cristina Setim Freitas; 2 André Twardowschy; 2 Ana Cristina dos Santos; 2 Bárbara Mayer; 1 Ana Paula Luiz; 2 Maria Consuelo Andrade Marques; 1 Adair Roberto Soares dos Santos;
1 Universidade Federal de Santa Catarina, Florianópolis, Brazil; 2 Universidade Federal do Paraná, Curitiba, Brazil
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| Content: | Pfaffia glomerata (Spreng) Pedersen (Amaranthaceae) is a medicinal plant known in Brazil as "Paratudo" and "Brazilian ginseng" and is commonly used as tonic, antidiabetic and to treat gastric disorders. Previous studiesshowed the anti-inflammatory and antinociceptive effects of the hydroalcoholic extract of roots from P. glomerata.
Objectives: The present study evaluated the possible mechanisms of action involved in the antinociceptive properties of the hydroalcoholic extract of roots from P. glomerata (EE70).
Materials and Methods: The nociceptive response was evaluated with acetic acid-induced writhings, formalin and glutamate induced-licking models. The opioid and glutamatergic systems were also evaluated.
Results: Oral administration of the EE70 (10-300 mg/kg) inhibited
the acetic acid-induced nociceptive response with ID50 of 64.5 +/- 10.7 mg/kg. In the formalin test, the EE70 at dose of 1000 mg/kg (p.o.) caused inhibition of 33% in neurogenic pain (early phase) but did not alter the inflammatory pain (late phase). The naloxone (5 mg/kg, i.p.) did not reverse the antinociception caused by EE70 (300 mg/kg, p.o.) in the acetic acid-induced pain test. The oral pre-treatment of the EE70 promoted a dose-related inhibition of the glutamate induced-licking response (ID50 of 363 +/- 16 mg/kg). When spinal excitatory amino acids (NMDA, AMPA, kainate and trans-ACPD) agonists and pro-inflammatory cytokines(IL-1b and TNFa) were intratechally injected, the EE70 (300 mg/kg, p.o.) inhibited the biting response induced by trans-ACPD in 32% and by TNF-a in 74%.
Conclusion: Collectively, these results show that in the antinociceptive properties of EE70 were involved the glutamatergic system and the pro-inflammatory cytokine, TNFa. |
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