| Abstract No.: | C-E3168 |
| Country: | Canada |
| | |
| Title: | THE EFFECTS OF HIGH-ENERGY DIET ON THE EXPRESSION OF GLUCOKINASE MRNA IN THE RAT BRAIN. |
| | |
| Authors/Affiliations: | 1 Anne-Marie Poulin; 1 Elena Dana Baraboi; 1 Denis Richard; 1 Elena Timofeeva*;
1 Université Laval, Québec, QC, Canada
|
| | |
| Content: | Objectives: Glucokinase (GK) is a glycolytic enzyme catalyzing the first step in glycolysis and playing a role in neuronal fuel-sensing pathway. Although the expression of GK is confined to the brain areas responsible for regulation of food intake and energy expenditure, the implication of central GK in energy balance regulation is not yet clearly determined. The present experiments were designed to study how the brain expression of GK mRNA is modified by long-term high-energy diet. Methods: Sprague Dawley rats were fed ad libitum (AL) a regular chow or high fat high carbohydrate diet (HFHC) during 13 weeks. On 14th week rats were sacrificed in AL feeding conditions or after 12 hours of food deprivation. The expression of GK mRNA in the brain was detected using in situ hybridization histochemistry. Results: Daily food intake was higher in rats fed a regular chow compared to HFHC-fed animals. However, because of higher energy density of ingested food, HFHC rats gained more body weight and fat mass than chow-fed groups. High-energy diet significantly increased the expression of GK mRNA in the dorsomedial and ventromedial hypothalamus, arcuate nucleus, paraventricular hypothalamic nucleus, subfornical organ, median preoptic nucleus and paraventricular thalamic nucleus in HFHC rats compared to chow-fed animals. Twelve hours of food deprivation had no effect on the brain GK mRNA expression in chow-fed rats but significantly decreased the levels of GK transcript in HFHC rats. Conclusion: The expression of brain GK mRNA is increased by long-term high-energy diet. This diet-induced increase in GK transcript is reversed by food deprivation. This result suggests that brain expression of GK is regulated according to metabolic status. |
| | |
| Back |
|
