| Abstract No.: | C-C3103 |
| Country: | Canada |
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| Title: | INFLUENCE OF DURATION OF HYPOTHERMIA ON OUTCOME AFTER PERMANENT MIDDLE CEREBRAL ARTERY OCCLUSION IN RAT |
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| Authors/Affiliations: | 1 Darren Clark*; 1 Mark Penner; 1 Ian Orellana-Jordan; 1 Frederick Colbourne;
1 University of Alberta, Edmonton, AB, Canada
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| Content: | Objectives: Hypothermia has provided significant neuroprotection in rodent models of ischemic brain damage. The ideal hypothermic profile has yet to be determined to treat acute stroke. In this study we examined the impact of differing hypothermic treatment durations on outcome after permanent middle cerebral artery occlusion (pMCAO). We assessed functional and histological recovery following 12, 24 and 48 of hypothermia beginning one hour after pMCAO, produced by cauterization of the distal MCA.
Materials and Methods: Histological and behavioral outcome was evaluated at one week after pMCAO. Brain water content (BWC) was measured in hypothermic groups at day 1 and 3 following pMCAO and normothermic animals were assessed on days 1 to 5 following injury.
Results: Hypothermia for 24 and 48 hours improved walking ability and all three hypothermia treatments reduced neurological deficit compared to controls. Only 24 and 48 hours of hypothermia reduced infarct size. BWC was elevated in the affected cortex for 5 days following stroke and for the first three days in the ipsilateral striatum. Hypothermia reduced BWC in the affected cortex and striatum on day 1 and in the ipsilateral striatum on day 3 following stroke.
Conclusion: Our results indicate that hypothermia, even when delayed, provides significant functional recovery and reduces edema and infarct size; histological and functional outcome is dependant on length of cooling.
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