| Abstract No.: | B-B2051 |
| Country: | Canada |
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| Title: | D1 RECEPTORS PHYSICALLY INTERACT WITH N-TYPE CALCIUM CHANNEL COMPLEXES TO REGULATE CHANNEL TRAFFICKING AND DENDRITIC CALCIUM ENTRY |
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| Authors/Affiliations: | 1 Alexandra Kisilevsky; 1 Christophe Altier*; 1 Mircea Iftinca1; 1 Diego Varela1; 1 Shahid Hameed1; 1 Gerald Zamponi1;
1 University of Calgary, AB, Canada
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| Content: | Dopamine signaling through D1 receptors in the prefrontal cortex (PFC) plays a critical role in the maintenance of higher cognitive functions such as working memory. At the cellular level, these functions are predicated to involve alterations in neuronal calcium levels. The dendrites of PFC neurons express both D1 receptors and N-type calcium channels, yet little information exists regarding D1 receptor-N-type channel coupling. Here, we show that D1 receptors potently inhibit N-type calcium channels in dendrites of rat PFC neurons. Using co-immunoprecipitation, we demonstrate the existence of a D1 receptor-N-type calcium channel signaling complex in this region. In addition, we provide evidence for a direct receptor-channel interaction via cytoplasmic regions of both proteins. Finally, we demonstrate the importance of this complex to receptor-channel co-trafficking in heterologous systems and in PFC neurons. Our data indicate that the N-type calcium channel is an important physiological target of D1 receptors and provide a novel mechanism for D1 receptor mediated regulation of cognitive function in the PFC. |
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