| Abstract No.: | A-B1067 |
| Country: | Canada |
| | |
| Title: | DISTINCT CLASSES OF INTERNEURONS EXPRESSING CALCIUM-BINDING PROTEINS INNERVATE THE LAYERS OF RAT PIRIFORM CORTEX |
| | |
| Authors/Affiliations: | 2 Cezar Gavrilovici*; 1 Sabrina D'Alfonso; 2 Michael Poulter;
1 Neuroscience Research Institute, Department of Psychology, Carleton University, Ottawa, ON, Canada; 2 Robarts Research Institute, Cell Biology Research Group, University of Western Ontario, ON, Canada
|
| | |
| Content: | The piriform cortex (PC) receives its primary sensory input from the lateral olfactory tract passing this neural information onto a number of cortical and subcortical structures where it is integrated with a number of behavioural modalities. The patterns innervation and high interconnectivity of the PC provide the pathways for sensory and behavioural olfactory responses; however these pathways may also be responsible for the region's high seizurogenic tendencies. Thus, understanding this wiring is important from both a physiological and pathophysiological perspective. In particular the understanding of the types of inhibitory interneurons and their innervation patterns is lacking. As functionally and morphologically distinct interneurons have been shown to express differing calcium binding proteins (CBPs): parvalbumin (PV), calbindin D28K (CB) and calretinin (CR), antibodies against these CBPs were used to characterize the laminar distribution of CBP expressing neurons, CBP co-localization, dendritic arborization patterns and the laminar distribution of nerve terminals (NT).
We found that only PV and CB are co-expressed in PC. Each CBP interneuron subtype demonstrated specific dendritic arborizations: PV-IR and PV/CB-IR cells showed equally wide, horizontal and vertical arborizations, CB-IR cells had wide horizontal and restricted vertical arborizations while CR-IR cells had restricted horizontal and very long vertical arborizations. Post-synaptic morphological targeting was also found to be specific, namely: PV-positive (+) NTs and PV/CB+ NTs innervate only perisomatic regions of principal cells; CR+ NTs innervate only dendrites of principal cells; and CB+ NTs innervate both somata and dendrites of principal cells. These data show for the first time the high complexity of the innervation pattern for all the CBP interneurons of PC and form a basis for further studies in the plasticity of this region.
|
| | |
| Back |
|
