| Abstract No.: | A-C1092 |
| Country: | Canada |
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| Title: | NEUROPROTECTIVE EFFECT OF 24-EPIBRASSINOLIDE ON 1-METHYL-4-PHENYL-PYRIDINE-INDUCED TOXICITY IN NEURONAL PC12 CELLS |
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| Authors/Affiliations: | 1 Julie Carange*; 1 Fanny Longpré; 1 Benoît Daoust; 1 Maria-Grazia Martinoli;
1 Université du Québec à Trois-Rivières, QC, Canada
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| Content: | Parkinson’s disease (PD) is a common and disabling neurodegenerative disease marked by progressive motor dysfunction, which results from selective degeneration of the nigrostriatal pathway. The etiology of PD remains unclear but there is growing evidence to suggest a role for oxydative damage in the pathogenesis of this disease. We have recently demonstrated the antioxydant role of a subset of natural polyphenols, such as resveratrol and quercetin.
Another class of plant hormones, the brassinosteroids, are steroidal plant hormones with chemical structures similar to animal steroidal hormones. The aim of our study was to verify the neuroprotective effect and the antioxydant potential of 24-epibrassinolide (24-epi), a common brassinosteroid, on the oxydative stress induced by 1-methyl-4-phenyl-pyridine (MPP+), a parkinsonism inducing toxin, in neuronal PC12 cells. First we investigated the effect of 24-epi on MPP+-induced cytotoxicity measured by using lactate deshydrogenase (LDH) colorimetric analysis. Then, we performed Western blot analysis to determine whether 24-epi might modulate the expression of tyrosine hydroxylase (TH), the precursor of dopamine, as well as the protein expression of Bax and Bcl-2, two members of the Bcl-2 family that play key roles in the regulation of apoptosis. Cytotoxicity measurements using LDH revealed a significant cell death following 24-hours exposure to MPP+. Preincubation with 24-epi prior to induction of oxidative stress attenuates the toxic effect of MPP+. Immunoblot analysis demonstrates that 24-epi increases TH protein expression in neuronal PC12 cells. Pretreatment with 24-epi also may modulate the expression of Bax and Bcl-2. In conclusion, our results demonstrate that 24-epi, a plant hormone, has a neuroprotective effect on MPP+-induced cytotoxicity in neuronal PC12 cells, thus it might be regarded as a powerful neuroprotective molecule.
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