TWO Graduate student positions in Neural Stem Cell Biology & Neural Stem Cell Regeneration – LINDSEY LAB – University of Manitoba

Graduate student position in Neural Stem Cell Regeneration

The Lindsey Lab is looking for a highly motivated student to fill an open funded graduate student position for September 2019. The focus of the project will be on the cellular and molecular regulation of neural stem cells during the regenerative process following CNS injury in the zebrafish model. The Lindsey Lab is the first laboratory at the University of Manitoba to establish the zebrafish model, and one of the only laboratories in Canada investigating CNS neural stem cell repair using this experimental system. Unlike in mammals, the zebrafish brain remains highly regenerative throughout life. This is made possible by distinct populations of neural stem cells with the potential to generate newborn neurons. The exceptional regenerative capacity of the zebrafish has made it a mainstream vertebrate model to uncover fundamental mechanisms of neural repair for the development of novel approaches to regenerative medicine. To date however, the interplay between the innate molecular programs of regenerative neural stem cells, the niche micro-environment, and the immune response, that together promote successful adult CNS regeneration is poorly understood. We are interested in characterizing the cellular and molecular regulators of neural stem cell populations post-injury to better understand how we may harness the regenerative potential of zebrafish neural stem cells for human therapies related to neurotrauma and neurodegenerative disease.

Graduate student position in Neural Stem Cell Biology

The Lindsey Lab is looking for a highly motivated student to fill an open funded graduate student position for September 2019. The focus of the project will be on neural stem cell niche development in the zebrafish model. The Lindsey Lab is the first laboratory at the University of Manitoba to establish the zebrafish model, and one of few laboratories in Canada investigating CNS neural stem cell development and plasticity using this experimental system. In recent years, the zebrafish has become a leading model for examining neural stem cell behavior and plasticity. The transparency of zebrafish larvae along with the abundance of lifelong stem cell compartments makes this model highly amenable to tracking stem cell niche growth, maintenance, and senescence.

The vertebrate adult neurogenic niche is composed of heterogeneous stem cell populations that receive cues from the vasculature, adjacent neurons, and ventricular cerebral spinal fluid. How adult neural stem cell niches of the vertebrate brain are constructed over development however, currently remains poorly understood. We are interested in defining how diverse stem cell niches are established from the larval to adult brain, and the cellular (i.e. cell cycle changes, differentiation, apoptosis, senescence) and molecular (i.e. signaling pathways, genes, growth factors) drivers involved in this process.

About the Lindsey Lab

The overarching research focus of the Lindsey Lab is to understand context-dependent behavior and control of distinct neural stem cell populations during development, with environmental interactions and learning, and repair. We use a combination of modern cellular and molecular approaches, such as proliferation and senescence assays, lineage tracing, in situ hybridization, transgenic animals, immunohistochemistry, RNA-sequencing, and injury models. These techniques are coupled with advanced 2-dimensional (live or fixed fluorescence confocal microscopy, electron microscopy and correlative electron microscopy) and 3-dimensional (optical projection tomography, confocal/light-sheet microscopy, tissue clearing) imaging. Finally, we use specialized behavioural software to understand the relationship between animal behavior/neurotrauma – stem cell dynamics – and neurogenesis/neuro-regeneration.

Students joining the Lindsey Lab can expect high quality research training and mentorship in a supportive and collaborative learning environment. Trainees will have the opportunity to present their work at local, national, and international scientific meetings and collaborate with diverse researchers within the Rady Faculty of Health Sciences. A highly competitive graduate student stipend is available for the successful candidate. However, all students will be expected to apply for local and national level graduate student scholarships during tenure of the position.

The Lindsey Lab is located within the Department of Human Anatomy and Cell Science in the Rady Faculty of Health Sciences at the University of Manitoba in Winnipeg. http://umanitoba.ca/faculties/health_sciences/medicine/units/anatomy/12818.html

How to apply

Interested candidates should send a brief statement of research interests, a copy of their most recent transcript, and their CV to Dr. Benjamin Lindsey at Benjamin.lindsey@umanitoba.ca. If shortlisted, potential candidates will be asked to provide contact information for three references. Students with an interest in fundamental neural stem cell behavior are highly encouraged to apply.